A-B) Flow cytometry of cerebrospinal fluid (CSF) derived cells (A) and peripheral blood mononuclear cells, PBMCs(B). Check it closer!
C ) Quantification of the percent of cells in the myeloid or lymphoid gates. View code
D ) Percent of myeloid cells that expressed P2RY12.
E ) Percent of myeloid cells co-expression CD14 and CD16.
F ) Percent of myeloid cells CD14+;CD16- View code for D-F
G-O ) UMAP of FACS data from all 6 subjects with cells from CSF or PBMC (H) and expression of and expression of CD3, CD20 or CD56 (I), CD11B (J), TLR2 (K), HLA-DR (L), P2RY12 (M), CD14 (N) and CD16 (O). Check it closer!
n= 6 paired CSF and PBMC samples analyzed using paired t-test. * p<0.05.